論文 - 小松　節子

Subcellular proteomics to understand promotive effect of plant-derived smoke solution on soybean root 招待 査読 国際共著 国際誌

Murashita Y, Nishiuchi T, Rehman SU, Komatsu S

Proteomes   9   39   2021年10月

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Plant-derived smoke solution enhances soybean root growth; however, its mechanism is not clearly understood. Subcellular proteomics techniques were used for underlying roles of plant-derived smoke solution on soybean root growth. The results indicate that plant-derived smoke solution enhanced soybean root growth through the transcriptional promotion with RNA polymerase II expression and the energy production with ATPase accumulation.

Proteomic and biological analyses reveal the effect on growth under flooding stress of chickpea irradiated with millimeter waves 査読 国際共著 国際誌

Komatsu S, Maruyama J, Furuya T, Yin X, Yamaguchi H, Hitachi K, Miyashita N, Tsuchida K, Tani M

J Proteome Res   20   4718 - 4727   2021年10月

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Millimeter wave irradiation has influences on organisms, and it improves the growth of plants such as soybean. To reveal the dynamic effects of millimeter-wave irradiation on chickpea under flooding, gel- and label-free proteomic analysis was conducted. The findings suggest that irradiation with millimeter waves on chickpea seeds improves the recovery of plant growth through regulation of photosynthesis in leaves and fermentation in roots. Furthermore, millimeter-wave irradiation might promote chickpea tolerance under flooding via the regulation of cell death.

Proteomic and biochemical analyses of the mechanism of tolerance in mutant soybean responding to flooding stress 招待 査読 国際誌

Komatsu S, Yamaguchi H, Hitachi K, Tsuchida K, Kono Y, Nishimura M

Int J Mol Sci   22   9046   2021年8月

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To investigate the mechanism of flooding tolerance of soybean, flooding-tolerant mutants derived from gamma-ray irradiated soybean were crossed with parent cultivar Enrei for removal of other factors besides the genes related to flooding tolerance in primary generated mutant soybean. The results suggest that the regulation of cell death through the fermentation system and glycoprotein folding might be an important factor for the acquisition of flooding tolerance in mutant soybean.

An information-theoretic model suggesting genetic regulatory system degradation under climate change 招待 査読 国際共著 国際誌

Sakata K, Saito T, Ohyanagi H, Katam R, Komatsu S

Physics Open   6   100062   2021年4月

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Does climate change influence on genetic regulatory systems? Previous studies on genetic regulatory systems have focused on specific systems and environmental stimuli. We elucidate a causal relationship for a ‘minimal’ genetic regulatory system in environmental changes. We manifest a mathematical framework for designing genetic modification strategy to mitigate the effect of the climate change on genetic regulatory systems. This model suggests genetic regulatory system degradation in the progression of climatic change

Osmotic stress in banana is relieved by exogenous nitric oxide 査読 国際共著 国際誌

Mohd Amnan MA, Pua TL, Lau SE, Tan BC, Yamaguchi H, Hitachi K, Tsuchida K, Komatsu S

PeerJ   9   10879   2021年4月

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Drought is one of the severe environmental stresses threatening agriculture around the globe. Nitric oxide plays diverse roles in plant growth and defensive responses. In this study, ptoteomic approach was used to determine the effects of sodium nitroprusside on polyethylene glycol-induced osmotic stress in banana roots. The results suggest that the nitric oxide-induced osmotic stress tolerance could be associated with improved carbohydrate and energy metabolism capability in higher plants.

Proteomic approaches to uncover salt stress response mechanisms in crops. 招待 査読 国際共著 国際誌

Kausar R, Komatsu S.

Int J Mol Sci   24   518   2023年1月

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Salt stress is an unfavorable outcome of global climate change, adversely affecting crop growth and yield. It is the second-biggest abiotic factor damaging the morphological, physiobiochemical, and molecular processes during seed germination and plant development. Salt responses include modulation of hormonal biosynthesis, ionic homeostasis, the antioxidant defense system, and osmoprotectants to mitigate salt stress. Plants trigger salt-responsive genes, proteins, and metabolites to cope with the damaging effects of a high salt concentration. Enhancing salt tolerance among crop plants is direly needed for sustainable global agriculture. Novel protein markers, which are used for crop improvement against salt stress, are identified using proteomic techniques. As compared to single-technique approaches, the integration of genomic tools and exogenously applied chemicals offers great potential in addressing salt-stress-induced challenges. The interplay of salt-responsive proteins and genes is the missing key of salt tolerance. The development of salt-tolerant crop varieties can be achieved by integrated approaches encompassing proteomics, metabolomics, genomics, and genome-editing tools. In this review, the current information about the morphological, physiological, and molecular mechanisms of salt response/tolerance in crops is summarized. The significance of proteomic approaches to improve salt tolerance in various crops is highlighted, and an integrated omics approach to achieve global food security is discussed. Novel proteins that respond to salt stress are potential candidates for future breeding of salt tolerance.

Crop proteomics under abiotic stress: from data to insights 招待 査読 国際共著 国際誌

Kausar R, Wang X, Komatsu S.

Plants   11 ( 21 )   2877   2022年10月

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Food security is a major challenge in the present world due to erratic weather and climatic changes. Environmental stress negatively affects plant growth and development which leads to reduced crop yields. Technological advancements have caused remarkable improvements in crop-breeding programs. Proteins have an indispensable role in developing stress resilience and tolerance in crops. Genomic and biotechnological advancements have made the process of crop improvement more accurate and targeted. Proteomic studies provide the information required for such targeted approaches. The crosstalk among cellular components is being analyzed by subcellular proteomics. Additionally, the functional diversity of proteins is being unraveled by post-translational modifications during abiotic stress. The exploration of precise cellular responses and the networking among different cellular organelles help in the prediction of signaling pathways and protein–protein interactions. High-throughput mass-spectrometry-based protein studies are now possible due to incremental advancements in mass-spectrometry techniques, sample protocols, and bioinformatic tools as well as the increasing availability of plant genome sequence information for multiple species. In this review, the key role of proteomic analysis in identifying the abiotic-stress-responsive mechanisms in various crops was summarized. The development and availability of advanced computational tools were discussed in detail. The highly variable protein responses among different crops have provided a wide avenue for molecular-marker-assisted genetic buildup studies to develop smart, high-yielding, and stress-tolerant varieties to cope with food-security challenges.

The role of phytohormones in plant response to flooding 招待 査読 国際共著 国際誌

Wang X, Komatsu S.

Int J Mol Sci   23 ( 12 )   6383   2022年7月

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Climatic variations influence the morphological, physiological, biological, and biochemical states of plants. Plant responses to abiotic stress include biochemical adjustments, regulation of proteins, molecular mechanisms, and alteration of post‐translational modifications, as well as signal transduction. Among the various abiotic stresses, flooding stress adversely affects the growth of plants, including various economically important crops. Biochemical and biological techniques, including proteomic techniques, provide a thorough understanding of the molecular mechanisms during flooding conditions. In particular, plants can cope with flooding conditions by embracing an orchestrated set of morphological adaptations and physiological adjustments that are regulated by an elaborate hormonal signaling network. With the help of these findings, the main objective is to identify plant responses to flooding and utilize that information for the development of flood‐tolerant plants. This review provides an insight into the role of phytohormones in plant response mechanisms to flooding stress, as well as different mitigation strategies that can be successfully administered to improve plant growth during stress exposure. Ultimately, this review will expedite marker assisted genetic enhancement studies in crops for developing high‐yield lines or varieties with flood tolerance.

Proteomic study to understand promotive effects of plant-derived smoke on soybean root growth under flooding stress 査読 国際共著

Otori M, Murashita Y, Regman S, Komatsu S.

Plant Mol. Biol Rep.   39   24 - 33   2021年3月

Proteomic analysis reveals the effects of melatonin on soybean root tips under flooding stress 査読

Wang X, Li F, Chen Z, Yang B, Komatsu S, Zhou S.

J Proteomics   230   104064   2021年3月

Quantitative proteomics reveals dual effects of calcium on radicle protrusion in soybean 査読

Wang X, Hu H, Li F, Yang B, Komatsu S, Zhou S.

J Proteomics   230   103999   2021年1月

Proteomics analysis of plasma membrane fractions of the root, leaf, and flower of rice 査読

Iwasaki Y, Itoh T, Hagi Y, Matsuta S, Nishiyama A, Chaya G, Kobayashi Y, Miura K, Komatsu S.

Int J Mol Sci221   21 ( 19 )   6988   2020年9月

A comparative proteomic analysis of engineered and bio synthesized silver nanoparticles on soybean seedlings 査読

Mustafa G, Hasan M, Yamaguchi H, Hitachi K, Tsuchida K, Komatsu S

J Proteomics   224   103833   2020年7月

An information-theoretic model for managing predator?prey ecosystems facing climate changes 招待 査読

Sakata K, Katam R, Saito T, Ohyanagi H, Komatsu S

International Journal on Advances in Life Sciences   12   10 - 15   2020年6月

Chitosan effects on the elevation of essential oils and antioxidant activity of Carum copticum L. seedlings and callus cultures under in vitro salt stress 査読

Razavizadeh R, Adabavazeh F .U344, Komatsu S.

Journal of Plant Biochemistry and Biotechnology.   29 ( 3 )   473 - 483   2020年6月

Plant-derived smoke enhances plant growth through ornithine-synthesis pathway and ubiquitin-proteasome pathway in soybean 査読

Zhong Z, Kobayashi T, Zhu W, Imai H. Zhao R, Ohno T, Rehman SU, Uemura M, Tian J, Komatsu S.

J Proteomics   221   103781   2020年6月

Proteomic analysis of irradiation with millimeter waves on soybean growth under flooding conditions 査読

Zhong Z, Furuya T, Ueno K, Yamaguchi H, Hitachi K, Tsuchida K, Tani M, Tian J, Komatsu S

Int J Mol Sci.   21 ( 2 )   486   2020年4月

Comparative proteomic analysis of wild-type physcomitrella patens and an OPDA-deficient physcomitrella patens mutant with disrupted PpAOS1 and PpAOS2 genes after wounding 査読

Luo W, Komatsu S, Abe T, Matsuura H, Takahashi K.

Int J Mol Sci.   21 ( 4 )   1417   2020年4月

Comparative analysis of the effect of inorganic and organic chemicals with silver nanoparticles on soybean under flooding stress 査読

Hashimoto T, Mustafa G, Nishiuchi T, Komatsu S.

Int J Mol Sci.   21 ( 4 )   1300   2020年4月

Phosphoproteomics reveals the biosynthesis of secondary metabolites in Catharanthus roseus under ultraviolet-b radiation. 査読

Zhong Z, Liu S, Zhu W, Ou Y, Yamaguchi H, Hitachi K, Tsuchida K, Tian J, Komatsu S.

J Proteome Res.   18   3328 - 2241   2019年9月