Updated on 2023/01/05

写真a

 
Yoshitaka Kimori
 

Degree

  • PhD   Coursework ( 2006.9   Kyushu Institute of Technology )

Research Areas

  • Informatics / Intelligent robotics

  • Informatics / Perceptual information processing

  • Life Science / Medical systems

Education

  • Kyushu Institute of Technology   Departmemt of Bioscience and Bioinformatics   Graduated

    1997.4 - 2001.3

  • Kyushu Institute of Technology   Master's Course   Completed

    2001.4 - 2003.3

  • Kyushu Institute of Technology   Major of Computer Science and Systems Engineering   Doctor's Course   Accomplished credits for doctoral program

    2003.4 - 2006.3

Research History

  • The University of Tokyo

    2006.4 - 2006.9

  • The University of Tokyo   The institute of medical science

    2006.10 - 2008.7

  • National center of neurology and psychiatry   National institute of Neuroscience   Researcher

    2008.8 - 2010.3

  • National institutes of natural sciences Center for Novel Science Initiatives

    2010.4 - 2011.9

  • National institutes of natural sciences Center for Novel Science Initiatives   Specially appointed assistant professor

    2011.10 - 2018.3

  • Fukui University of Technology   Associate Professor

    2018.4 - 2021.3

  • Fukui University of Technology   Professor

    2021.4

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Professional Memberships

  • IEEE

 

Papers

  • Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light Reviewed International journal

    Kazusato Oikawa, Shino Goto-Yamada, Yasuko Hayashi, Daisuke Takahashi, Yoshitaka Kimori, Michitaro Shibata, Kohki Yoshimoto, Atsushi Takemiya, Maki Kondo, Kazumi Hikino, Akira Kato, Keisuke Shimoda, Haruko Ueda, Matsuo Uemura, Keiji Numata, Yoshinori Ohsumi, Ikuko Hara-Nishimura, Shoji Mano, Kenji Yamada, Mikio Nishimura

    Nature Communications   13 ( 1 )   1 - 17   2022.12

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    Although light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. However, the cellular mechanism underlying ROS-induced leaf damage alleviation in peroxisomes was not fully explored. Here, we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We present evidence that autophagy-deficient mutants show light intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes. The peroxisome aggregates are specifically engulfed by pre-autophagosomal structures and vacuolar membranes in both leaf cells and isolated vacuoles, but they are not degraded in mutants. ATG18a-GFP and GFP-2×FYVE, which bind to phosphatidylinositol 3-phosphate, preferentially target the peroxisomal membranes and pre-autophagosomal structures near peroxisomes in ROS-accumulating cells under high-intensity light. Our findings provide deeper insights into the plant stress response caused by light irradiation.

  • A Morphological Image Preprocessing Method Based on the Geometrical Shape of Lesions to Improve the Lesion Recognition Performance of Convolutional Neural Networks Reviewed International journal

    Yoshitaka Kimori

    IEEE Access   10   70919 - 70936   2022.6

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    Convolutional neural networks (CNNs) play an important role in computer vision-related tasks for medical imaging. However, the quality of raw images in the dataset can be insufficient for the CNN model to learn the features of the target object. When the input image contains a complex background, the CNN model focuses on regions that are not essential for lesion recognition, such as background structures, leading to less accurate output prediction. This paper shows that this problem can be efficiently solved by an image preprocessing method based on mathematical morphology, which uses a priori knowledge about the lesion shape. The proposed method consists of h-dome transformation based on the geometrical shape information of the lesion region, and subsequent image histogram-modification processes, and has the ability to selectively enhance the lesion region from the background. This allows for the creation of images that explicitly represent the important region to be learned by the CNN model. Experiments on pulmonary nodule classification in chest x-ray images and skin lesion-region segmentation in dermatoscopic images demonstrate that the CNN models trained on the preprocessed dataset created by the proposed method achieve remarkable performance improvements compared to the CNN models trained on the original dataset.

  • Thermosensitive TRPV4 channels mediate temperature-dependent microglia movement Reviewed

    Rei Nishimoto, Sandra Derouiche, Kei Eto, Aykut Deveci, Makiko Kashio, Yoshitaka Kimori, Yoshikazu Matsuoka, Hiroshi Morimatsu, Junichi Nabekura, Makoto Tominaga

    Proceedings of the National Academy of Sciences   118 ( 17 )   e201289411   2021.4

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    Microglia maintain central nervous system homeostasis by monitoring changes in their environment (resting state) and by taking protective actions to equilibrate such changes (activated state). These surveillance and protective roles both require constant movement of microglia. Interestingly, induced hypothermia can reduce microglia migration caused by ischemia, suggesting that microglia movement can be modulated by temperature. Although several ion channels and transporters are known to support microglia movement, the precise molecular mechanism that regulates temperaturedependent movement of microglia remains unclear. Some members of the transient receptor potential (TRP) channel superfamily exhibit thermosensitivity and thus are strong candidates for mediation of this phenomenon. Here, we demonstrate that mouse microglia exhibit temperature-dependent movement in vitro and in vivo that is mediated by TRPV4 channels within the physiological range of body temperature. Our findings may provide a basis for future research into the potential clinical application of temperature regulation to preserve cell function via manipulation of ion channel activity.

  • Dynamic Capture-and-Release of Endoplasmic Reticulum Exit Sites by Golgi Stacks in Arabidopsis Reviewed

    Junpei Takagi, Yoshitaka Kimori, Tomoo Shimada, Ikuko Hara-Nishimura

    iScience   23 ( 7 )   101265   2020.7

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    Protein transport from the endoplasmic reticulum (ER) to Golgi stacks is mediated by the coat protein complex COPII, which is assembled at an ER subdomain called ER exit site (ERES). However, the dynamic relationship between ERESs and Golgi stacks is unknown. Here, we propose a dynamic capture-and-release model of ERESs by Golgi stacks in Arabidopsis thaliana. Using variable-angle epifluorescence microscopy with high-temporal-resolution imaging, COPII-component-bound ERESs were detected as punctate structures with sizes of 300–500 nm. Some punctate ERESs are distributed on ER tubules and sheet rims, whereas others gather around a Golgi stack in an ER-network cavity to form a beaded-ring structure. Free ERESs that wander into an ER cavity are captured by a Golgi stack in a cytoskeleton-independent manner. Then, they are released by the Golgi stack for recycling. The dynamic ERES cycling might contribute to efficient transfer of de novo synthesized cargo proteins from the ER to Golgi stacks.

  • A morphological image processing method to improve the visibility of pulmonary nodules on chest radiographic images. Reviewed

    Yoshitaka Kimori

    Biomedical Signal Processing and Control   57   101744   2020.3

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    A pulmonary nodular shadow on a chest radiographic image is characteristic of lung cancer. However, nodular shadows often appear with very weak contrast. The target nodule shadow often overlaps with a shadow with strong contrast, such as that of the heart or rib; thus, it is difficult to emphasize the target region with ordinary contrast enhancement methods. In this article, a new pulmonary nodule enhancement method using a morphological subtraction method based on a modification of the h-dome transform is described. This method consists of two parts: a preprocessing step and nodule enhancement processing. The former reduces the uneven brightness of the background, and the latter emphasizes the nodule. With this method, the pulmonary nodule region is emphasized with the use of shape information of the nodule, whereas emphasis of the regions surrounding the nodule with different shapes is suppressed. This method also detects the boundary of the nodule as well as the edges of the surrounding anatomically normal structures. The performance of the proposed method has been verified by nodule enhancement experiments using synthetic and actual chest radiographic images from a public database. Furthermore, the efficiency of proposed method was tested by using contrast improvement index (CII), which is an index of improvement in contrast in the nodule region and its surroundings, and the results were quantitatively compared with those obtained from existing contrast emphasis methods. The proposed method has the highest value of CII, thereby confirming the effect of the contrast emphasis with this method.

  • Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine. Reviewed

    Emi Hoshikawa, Taisuke Sato, Yoshitaka Kimori, Ayako Suzuki, Kenta Haga, Hiroko Kato, Koichi Tabeta, Daisuke Nanba, Kenji Izumi

    Journal of tissue engineering   10   2041731419881528   2019.10

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    Image-based cell/colony analyses offer promising solutions to compensate for the lack of quality control (QC) tools for noninvasive monitoring of cultured cells, a regulatory challenge in regenerative medicine. Here, the feasibility of two image analysis algorithms, optical flow and normalised cross-correlation, to noninvasively measure cell/colony motion in human primary oral keratinocytes for screening the proliferative capacity of cells in the early phases of cell culture were examined. We applied our software to movies converted from 96 consecutive time-lapse phase-contrast images of an oral keratinocyte culture. After segmenting the growing colonies, two indices were calculated based on each algorithm. The correlation between each index of the colonies and their proliferative capacity was evaluated. The software was able to assess cell/colony motion noninvasively, and each index reflected the observed cell kinetics. A positive linear correlation was found between cell/colony motion and proliferative capacity, indicating that both algorithms are potential tools for QC.

  • A Method for Evaluating Three-Dimensional Morphological Features: A Case Study Using Marchantia polymorpha Reviewed

    Tomoyuki Furuya, Yoshitaka Kimori, Hirokazu Tsukaya

    Frontiers in plant science   10   1214   2019.10

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    The description and evaluation of morphological features are essential to many biological studies. Bioimaging and quantification methods have been developed to analyze the morphological features of plants. However, efficient three-dimensional (3D) imaging and its quantification are still under development, particularly for studies of plant morphology, due to complex organ structure with great flexibility among individuals with the same genotype. In this study, we propose a new approach that combines a 3D imaging technique using micro-computed tomography and a mathematical image-processing method to describe 3D morphological features. As an example, we applied this method to Marchantia polymorpha, a new model plant used for the evolutional study of land plants, and we evaluated a mutant individual with an abnormal 3D shape. Using this new method, we quantitatively described the thallus morphology of M. polymorpha and distinguished the wild type from a mutant with different morphological features. Our newly established method can be applied to various tissues or bodies with irregular 3D morphology.

  • Autophagy controls reactive oxygen species homeostasis in guard cells that is essential for stomatal opening. Reviewed

    Shota Yamauchi, Shoji Mano, Kazusato Oikawa, Kazumi Hikino, Kosuke M Teshima, Yoshitaka Kimori, Mikio Nishimura, Ken-ichiro Shimazaki, Atsushi Takemiya

    Proceedings of the National Academy of Sciences   116 ( 38 )   19187 - 19192   2019.9

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    Reactive oxygen species (ROS) function as key signaling molecules to inhibit stomatal opening and promote stomatal closure in response to diverse environmental stresses. However, how guard cells maintain basal intracellular ROS levels is not yet known. This study aimed to determine the role of autophagy in the maintenance of basal ROS levels in guard cells. We isolated the Arabidopsis autophagy-related 2 (atg2) mutant, which is impaired in stomatal opening in response to light and low CO2 concentrations. Disruption of other autophagy genes, including ATG5, ATG7, ATG10, and ATG12, also caused similar stomatal defects. The atg mutants constitutively accumulated high levels of ROS in guard cells, and antioxidants such as ascorbate and glutathione rescued ROS accumulation and stomatal opening. Furthermore, the atg mutations increased the number and aggregation of peroxisomes in guard cells, and these peroxisomes exhibited reduced activity of the ROS scavenger catalase and elevated hydrogen peroxide (H2O2) as visualized using the peroxisome-targeted H2O2 sensor HyPer. Moreover, such ROS accumulation decreased by the application of 2-hydroxy-3-butynoate, an inhibitor of peroxisomal H2O2-producing glycolate oxidase. Our results showed that autophagy controls guard cell ROS homeostasis by eliminating oxidized peroxisomes, thereby allowing stomatal opening.

  • Heterogeneous distribution of doublecortin‐expressing cells surrounding the rostral migratory stream in the juvenile mouse Reviewed

    Yuka Aoyagi, Terumasa Hibi, Yoshitaka Kimori, Masato Sawada, Ryosuke Kawakami, Kazunobu Sawamoto, Tomomi Nemoto

    Journal of Comparative Neurology   526 ( 16 )   2631 - 2646   2018.11

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    In the postnatal mammalian brain, neural stem cells of the ventricular–subventricular zone continue to generate doublecortin (Dcx)-expressing immature neurons. Throughout life, these immature neurons migrate to the olfactory bulb through the rostral migratory stream (RMS). In this study, we investigated the distribution of these putative immature neurons using enhanced green fluorescent protein (EGFP) expression in the area surrounding the RMS of the juvenile Dcx-EGFP mice. Through the combined use of an optical clearing reagent (a 2,2′-thiodiethanol solution) and two-photon microscopy, we visualized three-dimensionally the EGFP-positive cells in the entire RMS and its surroundings. The resulting wide-field and high-definition images along with computational image processing methods developed in this study were used to comprehensively determine the position of the EGFP-positive cells. Our findings revealed that the EGFP-positive cells were heterogeneously distributed in the area surrounding the RMS. In addition, the orientation patterns of the leading process of these cells, which displayed the morphology of migrating immature neurons, differed depending on their location. These novel results provide highly precise morphological information for immature neurons and suggest that a portion of immature neurons may be detached from the RMS and migrate in various directions.

  • Endoplasmic Reticulum (ER) Membrane Proteins (LUNAPARKs) are Required for Proper Configuration of the Cortical ER Network in Plant Cells Reviewed

    Haruko Ueda, Natsumi Ohta, Yoshitaka Kimori, Teruka Uchida, Tomoo Shimada, Kentaro Tamura, Ikuko Hara-Nishimura

    Plant and Cell Physiology   59 ( 10 )   1931 - 1941   2018.10

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    The endoplasmic reticulum (ER) is a large network made of membranous cisternae and tubules, which accounts for a large proportion of the total lipid bilayer endomembrane of the cell. In mammals and yeast, LUNAPARK proteins are preferentially localized at the three-way junctions of the ER network, stabilizing the junctions and establishing the ER architecture. We identified two Arabidopsis homologs and designated them LNPA and LNPB. Subcellular localization analysis with a non-dimerizable type of green fluorescent protein (GFP) revealed that both LNPA and LNPB are predominantly distributed throughout the ER, but not preferentially localized at the three-way junctions. Quantitative analysis of the network in the double mutant lnpa lnpb revealed that deficiency of LNPA and LNPB caused the cortical ER to develop poor ER cisternae and a less dense tubular network. These phenotypes are opposite to those of LNP-deficient mutants of yeast and mammals. Despite the importance of cysteine residues in the zinc finger motif of the yeast LNP homolog (Lnp1p), the corresponding cysteine residues of LNPA were not necessary for the stabilization of ER morphology because replacing the four cysteine residues in the zinc finger motif of the LNPA protein with alanine residues did not affect its function. A significant phenotype of lnpa lnpb is generation of large spherical structures from the ER. Formation of the structures might reduce the amounts of the ER membrane to be used for generating the network, resulting in poor development of the ER network. Taken together, our results suggest that plant LNPs function differently from those in yeast and mammals: they function to distribute ER membranes appropriately throughout the cells.

  • Length of myelin internodes of individual oligodendrocytes is controlled by microenvironment influenced by normal and input‐deprived axonal activities in sensory deprived mouse models Reviewed

    Yasuyuki Osanai, Takeshi Shimizu, Takuma Mori, Nobuhiko Hatanaka, Yoshitaka Kimori, Kenta Kobayashi, Shinsuke Koyama, Yumiko Yoshimura, Atsushi Nambu, Kazuhiro Ikenaka

    Glia   66 ( 11 )   2514 - 2525   2018.9

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    本研究は，脳に分布するグリア細胞の一種である，オリゴデンドロサイト（稀突起グリア細胞）の髄鞘形成メカニズムを解明したものである．その機能を解明するためには，髄鞘構造に関する詳細な情報が必要であるが，顕微鏡を用いた観察では困難であった． 本研究では，蛍光顕微鏡像によって取得されたオリゴデンドロサイトの3Dデータに基づき，その髄鞘構造の可視化を実施し，機能発現機構の詳細を調べた．

  • ANGUSTIFOLIA contributes to the regulation of three-dimensional morphogenesis in the liverwort Marchantia polymorpha Reviewed

    Tomoyuki Furuya, Koro Hattori, Yoshitaka Kimori, Sakiko Ishida, Ryuichi Nishihama, Takayuki Kohchi, Hirokazu Tsukaya

    Development   145 ( 18 )   dev161398   2018.9

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    ANGUSTIFOLIA(AN)遺伝子は，シロイヌナズナにおいて葉の細胞の横幅方向への極性伸長等の制御に関与しており，その欠損株では，葉の形態に関し，野生型とは異なるいくつかの特有な表現型をもつ．ゼニゴケのAN遺伝子は，シロイヌナズナ中でのANと同等の機能をもつことが知られているが，その役割の詳細は未知である．ゼニゴケのANホモログのノックアウト変異体は，葉状体がねじれる等の表現型を呈す．本研究では，ANホモログの形態形成に関与する役割について調べた．

  • Implications of maintenance of mother-bud neck size in diverse vital processes of Saccharomyces cerevisiae Reviewed

    Current Genetics   1 - 15   2018.7

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    真核細胞のモデル系である出芽酵母では，ポストゲノム時代を迎えてフェノーム研究（表現型の網羅的研究）が盛んに行なわれるようになり，研究者の目に頼らない画像解析システムの開発の必要性が高まってきた．本手法では，大容量の画像中から酵母の領域を自動で検出し，母細胞から出芽する娘細胞の境界(bud neck)のサイズを定量的に記述する手法を開発し，そのサイズの維持が増殖等の細胞プロセスに重要であることを示した．

  • Different Activity Patterns in Retinal Ganglion Cells of TRPM1 and mGluR6 Knockout Mice Reviewed

    Haruki Takeuchi, Sho Horie, Satoru Moritoh, Hiroki Matsushima, Tesshu Hori, Yoshitaka Kimori, Katsunori Kitano, Yasuhiro Tsubo, Masao Tachibana, Chieko Koike

    BioMed Research International   2963232   2018.5

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    感覚受容に関わるTRP(transient receptor potential)familyの１つであるTRPM1は網膜に強く発現しており，ヒトTRPM1遺伝子の変異が完全型停止性夜盲症の原因になっていることが知られている．また，TRPM1は，網膜ON型双極細胞の樹状突起において，mGluR
    6(代謝型グルタミン酸受容体6)と共局在することが分かっている．本研究では，TRMP1とmGluR6ノックアウト(KO)マウスの網膜桿体ON型双極細胞の神経終末の構造解析を行い，TRMP1 KO網膜では，mGluR KO網膜よりもシナプス終末のサイズは有意に小さいことを見出した．このことは，桿体ON型双極細胞においては，mGluR6ではなく，TRMP1の欠損がシナプス終末の制術に影響を及ぼしていることを示唆している．

  • YTH-RNA-binding protein prevents deleterious expression of meiotic proteins by tethering their mRNAs to nuclear foci Reviewed

    Yuichi Shichino, Yoko Otsubo, Yoshitaka Kimori, Masayuki Yamamoto, Akira Yamashita

    eLife   7   e32155   2018.2

  • Rabies Virus‐Mediated Oligodendrocyte Labeling Reveals A Single Oligodendrocyte Myelinates Axons from Distinct Brain Regions Reviewed

    Yasuyuki Osanai, Takeshi Shimizu, Takuma Mori, Yumiko Yoshimura, Nobuhiko Hatanaka, Atsushi Nambu, Yoshitaka Kimori, Shinsuke Koyama, Kenta Kobayashi, Kazuhiro Ikenaka

    Glia   65   93 - 105   2017.1

  • Ultrastructure of Compacted DNA in Cyanobacteria by High-Voltage Cryo-Electron Tomography Reviewed

    Kazuyoshi Murata, Sayuri Hagiwara, Yoshitaka Kimori, Yasuko Kaneko

    Scientific Reports   6   34934   2016.10

  • Quantifying Morphological Features of Actin Cytoskeletal Filaments in Plant Cells Based on Mathematical Morphology Reviewed

    Yoshitaka Kimori, Kazumi Hikino, Mikio Nishimura, Shoji Mano

    Journal of Theoretical Biology   389   123 - 131   2016.1

  • Irradiation-injured Brain Tissues Can Self-Renew in The Absence of The Pivotal Tumor Suppressor p53 in The Medaka (Oryzias latipes) Embryo Reviewed

    Takako Yasuda, Yoshitaka Kimori, Kento Nagata, Kento Igarashi, Tomomi Watanabe-Asaka, Shoji Oda, Hiroshi Mitani

    Journal of Radiation Research   57   9 - 15   2016.1

  • Single-Cell Phenomics in Budding Yeast Reviewed

    Yoshikazu Ohya, Yoshitaka Kimori, Hiroki Okada, Shinsuke Ohnuki

    Molecular Biology of the Cell   26   3920 - 3925   2015.11

  • Morphological Image Processing for Quantitative Shape Analysis of Biomedical Structures: Effective Contrast Enhancement Reviewed

    Yoshitaka Kimori

    Journal of Synchrotron Radiation   20   848 - 853   2013.11

  • Novel Configuration of A Myosin II Transient Intermediate Analogue Revealed by Quick-Freeze Deep-Etch Replica Electron Microscopy Reviewed

    Yoshitaka KIMORI, Norio BABA, Eisaku KATAYAMA

    Biochemical Journal   450   23 - 35   2013.2

  • Gamma-ray irradiation promotes premature meiosis of spontaneously differentiating testis-ova in the testis of p53 deficient medaka (Oryzias latipes) Reviewed

    T Yasuda, S Oda, Z Li, Y Kimori, Y Kamei, T Ishikawa, T Todo, H Mitani

    Cell Death and Disease   3   e395   2012.10

  • Mathematical Morphology-based Approach to the Enhancement of Morphological Features in Medical Images Reviewed

    Yoshitaka Kimori

    Journal of Clinical Bioinformatics   1   33   2011.12

  • Myostatin-deficient medaka Exhibit a Double-muscling Phenotype with Hyperplasia and Hypertrophy, which Occur Sequentially During Post-hatch Development Reviewed

    Shin-ichi Chisada, Hiroyuki Okamoto, Yoshihito Taniguchi, Yoshitaka Kimori, Atsushi Toyoda, Yoshiyuki Sakaki, Shunichi Takeda, Yasutoshi Yoshiura

    Developmental Biology   359   82 - 94   2011.11

  • Fractal Dimension Analysis and Mathematical Morphology of Structural Changes in Actin Filaments Imaged by Electron Microscopy Reviewed

    Yoshitaka Kimori, Eisaku Katayama, Nobuhiro Morone, Takao Kodama

    Journal of Structural Biology   176   1 - 8   2011.10

  • Extended Morphological Processing: A Practical Method for Automatic Spot Detection of Biological Markers from Microscopic Images Reviewed

    Yoshitaka Kimori, Norio Baba, Nobuhiro Morone

    BMC Bioinformatics   11   373   2010.7

  • Procedure to Analyze Surface Profiles of the Protein Molecules Visualized by Quick-Freeze Deep-Etch Replica Electron Microscopy Reviewed

    Yoshitaka Kimori, Yosuke Oguchi, Norihiko Ichise, Norio Baba, Eisaku Katayama

    Ultramicroscopy   107   25 - 39   2007.1

  • Aggregation of Partially Unfolded Myosin Subfragment-1 into Spherical Oligomers with Amyloid-Like Dye-Binding Properties Reviewed

    Hideyuki Komatsu, Nami Shinotani, Yoshitaka Kimori, Jun-ichiro Tokuoka, Kuniyoshi Kaseda, Hiroyuki Nakagawa, Takao Kodama

    Journal of Biochemistry   139   989 - 996   2006.6

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Books

  • Using image-based resources: databases for plant organelle dynamics, and applications based on image information,　In Introduction to Sequence and Genome Analysis I

    Mano S., Kimori Y., Takeda T., Miwa T., Nishikawa S., Mimura T.（ Role： Joint author ,  第3章「Morphological Image Processing for the Quantitative Analysis of Biological Images」, pp. 90-99.）

    iConcept Press  2013.7 

Presentations

  • Quantitative measurement of mobility of cell colony using image analysis methods for quality control of oral keratinocytes: A preliminary study International conference

    Hoshikawa E., Kimori Y., Sato T., Kato H., Suzuki A., Haga K., Nanba D., Izumi K.

    5th Tissue Engineering and Regenerative Medicine International Society World Congress  Tissue Engineering and Regenerative Medicine International Society

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    本研究は非侵襲的・定量的に測定した細胞運動能の指標と口腔粘膜上皮細胞の幹細胞特性との相関性を明らかにすることを目的とする．このため運動能測定に基づく細胞評価システムの構築をめざす．本発表で，患者に移植される自家細胞の品質管理を非侵襲的に行う，2つの画像解析手法正規化相互相関法とオプティカルフロー法）を提案し，その結果に基づき，細胞移動量に反映される細胞運動能と増殖能には強い相関があることを示した．